Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform. 

Kutys, M.L.*, Polacheck, W.J.*, Welch, M.K., Gagnon, K.A., Koorman, T., Kim, S., Li, L., McClatchey, A.I., Chen, C.S. 

Nature Communications


DOI: 10.1038/s41467-020-17102-x

Microfluidics for the study of mechanotransduction.

Griffith, C.M.*, Huang, S.A.*, Cho, C., Khare, T.M., Rich, M., Lee, G., Ligler, F.S., Diekman, B.O., Polacheck, W.J.

Journal of Physics Part D: Applied Physics


Functional angiogenesis requires microenvironmental cues balancing endothelial cell migration and proliferation. 

Wang, W.Y., Lin, D., Jarman, E.H., Polacheck, W.J., Baker, B.M.

Lab on a Chip


DOI: 10.1039/C9LC01170F


Collective tumor cell migration in the presence of fibroblasts.

Waldeland, J.O., Polacheck, W.J., Evje, S.

Journal of Biomechanics


PMID: 31902609, DOI: 10.1016/j.jbiomech.2019.109568

Photodynamic therapy and the biophysics of the tumor microenvironment.

Sorrin, A.J., Ruhi, M.K., Ferlic, N., Karimnia, V., Polacheck, W.J., Celli, J.P., Huang, H.C., Rizvi, I.R.

Photochemistry and Photobiology

96(2), 232-259.

PMID: 31895481, DOI: 10.1111/php.13209

A bile duct-on-a-chip with organ-level functions.

Du, Y., Khandekar, G., Llewellyn, J., Polacheck, W.J., Chen, C.S., Wells, R.G.



PMID: 31465556, PMCID: PMC7048662, DOI: 10.1002/hep.30918

Microfabricated blood vessels for modeling the vascular transport barrier.

Polacheck, W.J., Kutys, M.L., Tefft, J.B., Chen, C.S.

Nature Protocols

14, 1425-1454.

  • Featured on the cover. 


Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis.

Benhamouche, S., O’Loughlin, E., Liu, C., Polacheck, W.J., Fitament, J., El-Bardeesy, N., Chen, C.S., McClatchey, A.I. 


145(9), 1-11.

Force generation via β-cardiac myosin, titin, and α-actinin drives cardiac sarcomere assembly from focal adhesions.

Chopra, A. Kutys, M.L., Zhang, K., Polacheck, W.J., Sheng, C., Eyckmans, J., Seidman, J.G., Seidman, C.E., Hinson, J.T., Chen, C.S.

Developmental Cell



A non-canonical Notch signaling complex regulates adherens junctions and endothelial barrier function.

Polacheck, W.J., Kutys, M.L., Yang, J., Eyckmans, J.E., Wu, Y., Vasavada, H., Hirschi, K.K., and Chen, C.S.



Three-dimensional biomimetic vascular model reveals a RhoA, Rac1, and N-cadherin balance in mural cell-endothelial cell-regulated barrier function.

Alimperti, S., Mirabella, T., Bajaj, V., Polacheck, W.J., Ward, D., Duffield, J.D., Assoian, R., Chen, C.S. 

Proc. Nat. Acad. Sciences USA

2017, 114(33):8758-8763.

Matrix degradability controls multicellularity of 3D cell migration.

Trappmann, B., Baker, B.M., Polacheck, W.J., Choi, C.K., Burdick, J.A., Chen, C.S. 

Nature Communications

2017, 8(1):371

A microfluidics assay to study invasion of human placental trophoblast cells.

Abbas, Y., Oefner, C.M., Polacheck, W.J., Gardner, L., Farrell, L., Sharkey, A., Kamm, R.D., Moffett, A., Oyen, M.L

J. R. Soc. Interface

2017, 14(13): 20170131.

Inhibition of avb5 integrin leads to a pericyte-dependent attenuation of vascular permeability and protects against renal ischemia reperfusion injury.

McCurley, A., Alimperti, S., Campos-Bilderback, S.B., Sandoval, R.M., Calvino, J.E., Reynolds, T.L., Quigley, C., Mugford, J., Polacheck, W.J., Dovey, J., Marsh, G., Huang, A., Qian, F., Wienreb, P.H., Dolinski, B.M., Moore, S., Duffield, J.S., Chen, C.S., Molitoris, B.A., Violette, S.M., Crackower, M.A.

J. Am. Soc. Nephrol.

2017, 28(6):1741-1752.


Measuring cell-generated forces: a guide to the available tools.

Polacheck, W.J. and Chen, C.S. 

Nature Methods

2016, 13(5): 415-423.

Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.

Hinson, J.T., Chopra, A.C., Nafissi, N., Polacheck, W.J., Benson, C.C., Swist, S., Gorham, J., Yang, L., Schafer, S., Hubner, N., Church, G., Cook, S.A., Linke, W.A., Chen, C.S., Seidman, J.G., Seidman, C.E.


2015, 349 (6251): 982-986

Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy.

Scarcelli, G., Polacheck, W.J., Nia, H.T., Grodzinsky, A.J., Kamm, R.D., Yun, S.H.

Nature Methods

2015, 12(12): 1132-1134

 Mechanotransduction of fluid stresses governs 3D cell migration.

Polacheck, W.J., German, A.E., Mammoto, A., Ingber, D.E., and Kamm, R.D.

Proc. Nat. Acad. Sciences USA

2014, 111(7): 2447-2452

Probabilistic voxel-FE model for single cell motility in 3D.

Borau, C., Polacheck, W.J., Kamm, R.D., Garcia-Aznar, J.M. 

In Silico Cell and Tissue Science

2014, 1 (1), 2

Responsible research in an international laboratory: personal perspectives from a lab member and principal investigator.

Polacheck, W.J. and Kamm, R.D. 

J. Philosophy Science and Law

2014, 14:13-31

Microfluidic platforms for mechanobiology.

Polacheck, W.J., Li, R., Uzel, S.U., and Kamm, R.D.

Lab on a Chip

2013, 13(12):2252-2267

Tumor cell migration through complex microenvironments.

Polacheck, W.J., Zervantonakis, I.K., and Kamm, R.D.

Cell Mol. Life Sci.

2013, 70(8): 1335-1356

Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting.

Chan, J.M., Zervantonakis, I.K., Rimchala, T., Polacheck, W.J., Whisler, J., and Kamm, R.D.


2013, 7(12): e508

Interstitial flow influences tumor cell migration through competing mechanisms.

Polacheck, W.J., Charest, J.L., and Kamm, R.D.

Proc. Nat. Acad. Sciences USA

2011, 108(27): 11115-11120

Stiffness of Photocrosslinked RGD-Alginate Gels Regulates Adipose Progenitor Cell Behavior.

Chandler, E.M., Berglund, C.M., Lee, J.S., Polacheck, W.J., Gleghorn, J.P., Kirby, B.J., and Fischbach, C. 

Biotechnology and Bioengineering

2011, 108(7): 1683-1692

Methods for photocrosslinking alginate hydrogel scaffolds with high cell viability.

Rouillard, A.D., Berglund, C.M., Lee, J.Y., Polacheck, W.J., Tsui, Y., Bonassar, L.J., and Kirby, B.J. 

Tissue Engineering

2010, 17(2): 173-179

Control of the Electromechanical Properties of Alginate Tissue Scaffolds via Ionic and Covalent Crosslinking and Microparticle Doping.

Rouillard, A.D., Tsui, Y., Polacheck, W.J., Lee, J.Y., Bonassar, L.J., and Kirby, B.J.


2010, 11(8): 2184-2189