Publications

2019

Microfabricated blood vessels for modeling the vascular transport barrier.

Polacheck, W.J., Kutys, M.L., Tefft, J.B., Chen, C.S.

Nature Protocols

14, 1425-1454.

Featured on the cover.

2018

Proliferation-independent role of NF2 (merlin) in limiting biliary morphogenesis.

Benhamouche, S., O’Loughlin, E., Liu, C., Polacheck, W.J., Fitament, J., El-Bardeesy, N., Chen, C.S., McClatchey, A.I.

Development

145(9), 1-11.

Force generation via β-cardiac myosin, titin, and α-actinin drives cardiac sarcomere assembly from focal adhesions.

Chopra, A. Kutys, M.L., Zhang, K., Polacheck, W.J., Sheng, C., Eyckmans, J., Seidman, J.G., Seidman, C.E., Hinson, J.T., Chen, C.S.

Developmental Cell

44(1):87-96

2017

A non-canonical Notch signaling complex regulates adherens junctions and endothelial barrier function.

Polacheck, W.J., Kutys, M.L., Yang, J., Eyckmans, J.E., Wu, Y., Vasavada, H., Hirschi, K.K., and Chen, C.S.

Nature

552:258-262

Reviewed in a Current Biology dispatch entitled Vascular Permeability: Flow-Mediated, Non-canonical Notch Signalling Promotes Barrier Integrity.
Reviewed as a spotlight in Trends in Cell Biology entitled Notching a New Pathway in Vascular Flow Sensing.
Highlighted in Science Signaling editor's choice - cell and molecular signaling.
Highlighted in Faculty of 1000 - Cell Biology.
Highlighted in The Wyss Institute press release.

Three-dimensional biomimetic vascular model reveals a RhoA, Rac1, and N-cadherin balance in mural cell-endothelial cell-regulated barrier function.

Alimperti, S., Mirabella, T., Bajaj, V., Polacheck, W.J., Ward, D., Duffield, J.D., Assoian, R., Chen, C.S.

Proc. Nat. Acad. Sciences USA

2017, 114(33):8758-8763.

Matrix degradability controls multicellularity of 3D cell migration.

Trappmann, B., Baker, B.M., Polacheck, W.J., Choi, C.K., Burdick, J.A., Chen, C.S.

Nature Communications

2017, 8(1):371

A microfluidics assay to study invasion of human placental trophoblast cells.

Abbas, Y., Oefner, C.M., Polacheck, W.J., Gardner, L., Farrell, L., Sharkey, A., Kamm, R.D., Moffett, A., Oyen, M.L

J. R. Soc. Interface

2017, 14(13): 20170131.

Inhibition of avb5 integrin leads to a pericyte-dependent attenuation of vascular permeability and protects against renal ischemia reperfusion injury.

McCurley, A., Alimperti, S., Campos-Bilderback, S.B., Sandoval, R.M., Calvino, J.E., Reynolds, T.L., Quigley, C., Mugford, J., Polacheck, W.J., Dovey, J., Marsh, G., Huang, A., Qian, F., Wienreb, P.H., Dolinski, B.M., Moore, S., Duffield, J.S., Chen, C.S., Molitoris, B.A., Violette, S.M., Crackower, M.A.

J. Am. Soc. Nephrol.

2017, 28(6):1741-1752.

Older

Measuring cell-generated forces: a guide to the available tools.

Polacheck, W.J. and Chen, C.S.

Nature Methods

2016, 13(5): 415-423.

Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.

Hinson, J.T., Chopra, A.C., Nafissi, N., Polacheck, W.J., Benson, C.C., Swist, S., Gorham, J., Yang, L., Schafer, S., Hubner, N., Church, G., Cook, S.A., Linke, W.A., Chen, C.S., Seidman, J.G., Seidman, C.E.

Science

2015, 349 (6251): 982-986

Noncontact three-dimensional mapping of intracellular hydro-mechanical properties by Brillouin microscopy.

Scarcelli, G., Polacheck, W.J., Nia, H.T., Grodzinsky, A.J., Kamm, R.D., Yun, S.H.

Nature Methods

2015, 12(12): 1132-1134

Mechanotransduction of fluid stresses governs 3D cell migration.

Polacheck, W.J., German, A.E., Mammoto, A., Ingber, D.E., and Kamm, R.D.

Proc. Nat. Acad. Sciences USA

2014, 111(7): 2447-2452

Probabilistic voxel-FE model for single cell motility in 3D.

Borau, C., Polacheck, W.J., Kamm, R.D., Garcia-Aznar, J.M.